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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
30/01/2002 |
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Data da última atualização: |
31/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, L. de C.; CHIPPARI-GOMES, A. R.; LOPES, N. P.; ROUBACH, R.; ARAUJO-LIMA, C. A. R. M. |
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Afiliação: |
LEVY DE CARVALHO GOMES, CPAA. |
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Título: |
Efficacy of benzocaine as an anesthetic in juvenile tambaqui Colossoma macropomum. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Journal of the World Aquaculture Society, v. 32, n. 4, p. 426-431, 2001. |
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Idioma: |
Inglês |
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Conteúdo: |
The present study investigated the use of benzocaine as an anesthetic for juvenile Colossoma macropomum (tambaqui). In the first experiment, fish were exposed to various doses of benzocaine for 10 min at 24 C. There was no effect of temperature on the time required for fish to dose equilibrium. |
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Palavras-Chave: |
Anaesthesia; Anaesthetics; Methods. |
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Thesagro: |
Anestesia; Anestésico; Aquicultura; Colossoma Macropomum; Efeito Colateral; Medicamento; Método de Pesquisa; Piscicultura; Tambaqui; Temperatura. |
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Thesaurus Nal: |
aquaculture; drugs; fish culture; research; temperature. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01322naa a2200385 a 4500
001 1665668
005 2022-03-31
008 2001 bl uuuu u00u1 u #d
100 1 $aGOMES, L. de C.
245 $aEfficacy of benzocaine as an anesthetic in juvenile tambaqui Colossoma macropomum.
260 $c2001
520 $aThe present study investigated the use of benzocaine as an anesthetic for juvenile Colossoma macropomum (tambaqui). In the first experiment, fish were exposed to various doses of benzocaine for 10 min at 24 C. There was no effect of temperature on the time required for fish to dose equilibrium.
650 $aaquaculture
650 $adrugs
650 $afish culture
650 $aresearch
650 $atemperature
650 $aAnestesia
650 $aAnestésico
650 $aAquicultura
650 $aColossoma Macropomum
650 $aEfeito Colateral
650 $aMedicamento
650 $aMétodo de Pesquisa
650 $aPiscicultura
650 $aTambaqui
650 $aTemperatura
653 $aAnaesthesia
653 $aAnaesthetics
653 $aMethods
700 1 $aCHIPPARI-GOMES, A. R.
700 1 $aLOPES, N. P.
700 1 $aROUBACH, R.
700 1 $aARAUJO-LIMA, C. A. R. M.
773 $tJournal of the World Aquaculture Society$gv. 32, n. 4, p. 426-431, 2001.
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Embrapa Amazônia Ocidental (CPAA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/01/2022 |
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Data da última atualização: |
20/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
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Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; DAVID VILLAR-ÁLVAREZ, CSIC; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, CSIC; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, CSIC. |
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Título: |
Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Viruses , v.13, n.12, 2021. |
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ISSN: |
1999-4915 |
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DOI: |
https://doi.org/10.3390/v13122498 |
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Idioma: |
Inglês |
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Conteúdo: |
Previous results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements. |
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Thesagro: |
Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02107naa a2200217 a 4500
001 2139210
005 2022-01-20
008 2021 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $ahttps://doi.org/10.3390/v13122498$2DOI
100 1 $aLEASTRO, M. O.
245 $aSpontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage.$h[electronic resource]
260 $c2021
520 $aPrevious results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements.
650 $aVírus
700 1 $aVILLAR-ÁLVAREZ, D.
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
700 1 $aPALLÁS, V.
700 1 $aSÁNCHEZ-NAVARRO, J. Á.
773 $tViruses$gv.13, n.12, 2021.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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